Here are the locations and seedings for the NAIA Opening Round tournaments, to be played May 10-14:
| Bracket | Team |
| Paducah Bracket | No. 1 LSU-Shreveport |
| Hosted by Paducah Convention & Visitors Bureau - Paducah, Ky. | No. 2 Lindsey Wilson (KY) |
| No. 3 Bellevue (NE) | |
| No. 4 Peru State (NE) | |
| No. 5 Grand View (IA) | |
| Cleveland Bracket | No. 1 Lee (TN) |
| Hosted by Lee University (Tenn.) - Cleveland, Tenn. | No. 2 Freed-Hardeman (TN) |
| No. 3 Missouri Baptist | |
| No. 4 Avila (MO) | |
| No. 5 Bacone (OK) | |
| Oklahoma City Bracket | No. 1 Oklahoma City |
| Hosted by Oklahoma City University - Oklahoma City, Okla. | No. 2 Lubbock Christian (TX) |
| No. 3 Sterling (KS) | |
| No. 4 York (NE) | |
| No. 5 Mount Marty (SD) | |
| Azusa Bracket | No. 1 Azusa Pacific (CA) |
| Hosted by Azusa Pacific University (Calif.) - Azusa, Calif. | No. 2 Shorter (GA) |
| No. 3 College of Idaho | |
| No. 4 Arizona Christian | |
| No. 5 Menlo (CA) | |
| Hardeeville Bracket | No. 1 Faulkner (AL) |
| Hosted by University of South Carolina Beaufort - Hardeeville, S.C. | No. 2 St. Thomas (FL) |
| No. 3 South Carolina-Beaufort | |
| No. 4 Union (TN) | |
| No. 5 Asbury (KY) | |
| Kingsport Bracket | No. 1 Tennessee Wesleyan |
| Hosted by Appalachian Athletic Conference - Kingsport, Tenn. | No. 2 Georgetown (KY) |
| No. 3 Indiana Tech | |
| No. 4 Campbellsville (KY) | |
| No. 5 St. Francis (IL) | |
| Hutchinson Bracket | No. 1 Oklahoma Baptist |
| Hosted by Tabor College (KS) - Hillsboro, Kan. | No. 2 Judson (IL) |
| No. 3 Rogers State (OK) | |
| No. 4 Tabor (KS) | |
| No. 5 Dakota State (SD) | |
| Daytona Beach Bracket | No. 1 Embry-Riddle (FL) |
| Hosted by Embry-Riddle Aeronautical University (Fla.) - Daytona Beach, Fla. | No. 2 Auburn Montgomery (AL) |
| No. 3 Biola (CA) | |
| No. 4 Southern Poly (GA) | |
| No. 5 Siena Heights (MI) | |
| Marion Bracket | No. 1 Concordia (CA) |
| Hosted by Mid-Central College Conference - Marion, Ind. | No. 2 Point Park (PA) |
| No. 3 Taylor (IN) | |
| No. 4 Mount Vernon Nazarene (OH) | |
| No. 5 Doane (NE) | |